human tgf β1 htgf β1 induction  (MedChemExpress)

Journal: Inflammation

Article Title: A Novel Insight into Chronic Pancreatitis Pathogenesis: the USP1/ITGB5 Axis-Mediated Stellate Cell Activation

doi: 10.1007/s10753-025-02434-x

Figure Lengend Snippet: Proteomics reveals impairment of ECM degradation in PSCs with USP1 knockdown. A , B . USP1 expression was estimated in PSCs treated with different concentration of TGF-β1 (n = 3). C , D . The USP1 expression after lentiviral transfection was detected by real-time PCR ( C ) and western blot ( D ) ( n = 3). E Heap map of differentially expressed proteins (DEPs) in label-free proteomic ( n = 4). F . GO enrichment analysis of DEPs associated with collagen fibers. G . Heat map of collagen protein (n = 4). H . The protein expression of COL1A1, COL1A2, and FN in the PSCs was determined by western blot ( n = 3). I . Immunofluorescence staining of COL1A1 in the PSCs ( n = 3). Bar: 50 μm. J . Immunofluorescence staining of α-SMA. Bar: 50 μm. K . Quantitative analysis of the fluorescence intensity of COL1A1 ( I ) and α-SMA (J) ( n = 3). **, p < 0.01; ***, p < 0.001

Article Snippet: For human TGF-β1 (hTGF-β1) induction, cells were treated with 5 ng/mL hTGF-β1 (HY- P78668 , MCE, New Jersey, USA) for 0, 12, 24, or 48 h. For lentiviral infection, cells were cultured in virus-containing medium for 48 h; subsequently, infected cells were treated with 5 ng/mL hTGF-β1 for 24 h for further analysis.

Techniques: Knockdown, Expressing, Concentration Assay, Transfection, Real-time Polymerase Chain Reaction, Western Blot, Immunofluorescence, Staining, Fluorescence

Journal: Inflammation

Article Title: A Novel Insight into Chronic Pancreatitis Pathogenesis: the USP1/ITGB5 Axis-Mediated Stellate Cell Activation

doi: 10.1007/s10753-025-02434-x

Figure Lengend Snippet: USP1 knockdown promotes ITGB5 ubiquitination-mediated degradation. ( A ) Venn plot showing a total of 2015 proteins bind to USP1 under TGF-β1 stimulation. (B) KEGG pathway analysis and GO enrichment analysis pair of 2015 proteins in ( A ). ( C ) Label-free proteomics combined with IP-LC/MS to analysis the USP1 downstream target. The intersection of differential downregulated expressed protein of label-free proteomics, IP-LC/MS, and the upregulated genes of GSE41418 dataset. ( D ) The ITGB5-associated PPI was established via the String database. E-F. The expression of ITGB5 of the PSCs (E) ( n = 3) and pancreatic tissues ( F ) ( n = 6) was estimated by western blot. G . Co-IP shows the interaction of USP1 and ITGB5 in the PSCs ( n = 3). H . Ubiquitination detection of ITGB5 in the PSCs were measured by Co-IP ( n = 3). **, p < 0.01; ***, p < 0.001.

Article Snippet: For human TGF-β1 (hTGF-β1) induction, cells were treated with 5 ng/mL hTGF-β1 (HY- P78668 , MCE, New Jersey, USA) for 0, 12, 24, or 48 h. For lentiviral infection, cells were cultured in virus-containing medium for 48 h; subsequently, infected cells were treated with 5 ng/mL hTGF-β1 for 24 h for further analysis.

Techniques: Knockdown, Ubiquitin Proteomics, Liquid Chromatography with Mass Spectroscopy, Expressing, Western Blot, Co-Immunoprecipitation Assay